rabbit anti fshr primary antibody  (Bioss)

Journal: Frontiers in Endocrinology

Article Title: Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization

doi: 10.3389/fendo.2023.1095031

Figure Lengend Snippet: Immunohistochemical analysis of FFPE human premenopausal ovary, testis and skin tissue ( A–C , respectively). Polyclonal anti-FSHR antibody was ommitted (A1, B1 and C1) or the sections were incubated with the antibody (A2-3, B2-4, C2-3 in 1:500 dilution). Sections that were not incubated with the anti-FSHR antibody only showed blue nuclear staining. The asterisks show FSHR positive (red/brown) granulosa cells (A3) and possibly FSHR positive Sertoli cells among other positive cells in the seminiferous tubules of the testis (B3, B4). Staining inhibin in ovary tissue reveals how specific granulosa staining can be (A4). Staining of skin tissue with anti-cytokeratin 17 antibody shows specific staining of a hair follicle with its sebaceous gland (C4), as opposed to the aspecific staining throughout the skin tissue section by the anti-FSHR antibody. Magnification: Panels A1-2, B1-2, and C1-2 12.5x; Panels A3-4, B3-4, and C3-4 200x; Panel C4 100x.

Article Snippet: The sections were incubated with either the primary mouse monoclonal antibody directed against the human FSHR (clone FSHR/1400, NSJ Bioreagents; unclear which part of the FSHR it recognizes) or the primary rabbit anti-FSHR polyclonal antibody (MBS178821, MyBioSource; raised against the C18-N187 peptide sequence) in Normal Antibody Diluent (ABD999, Immunologic).

Techniques: Immunohistochemical staining, Incubation, Staining

Journal: Frontiers in Endocrinology

Article Title: Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization

doi: 10.3389/fendo.2023.1095031

Figure Lengend Snippet: Crystallographic image of the extracellular domain of the FSHR (blue, beige, yellow) folding over its ligand FSH (orange), adapted from Jiang et al. . In yellow the beta strands/leucine-rich repeat domains in amino acid stretch C18-N187 are highlighted, against which the polyclonal anti-FSHR antibody MBS178821 is raised. The blue ribbon represent the amino acids that exon 9 codes for, which lacks in the short FSHR variant.

Article Snippet: The sections were incubated with either the primary mouse monoclonal antibody directed against the human FSHR (clone FSHR/1400, NSJ Bioreagents; unclear which part of the FSHR it recognizes) or the primary rabbit anti-FSHR polyclonal antibody (MBS178821, MyBioSource; raised against the C18-N187 peptide sequence) in Normal Antibody Diluent (ABD999, Immunologic).

Techniques: Variant Assay

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Function in Modulating Human Granulosa Cell Steroidogenesis and Female Fertility

doi: 10.3390/ijms21103592

Figure Lengend Snippet: Mitochondrial function in modulating human granulosa cell steroidogenesis and female fertility. Cholesterol transferred from granulosa cells, by low-density lipoprotein (LDL) receptor-mediated endocytosis, into theca cells, where it is used as a substrate for steroidogenesis. The conversion of cholesterol to pregnenolone is initiated by the binding of luteinizing hormone (LH) to the LH receptor (LHR), and subsequent conversion of androgens to E2 is initiated by the binding of follicle-stimulating hormone (FSH) to the follicle-stimulating hormone receptor (FSHR). Mitochondria are the central sites for steroid hormone biosynthesis. The first step in the biosynthesis of steroid hormones is the transfer of cholesterol to the mitochondrial outer membrane, which is facilitated by StAR. Then, cytochrome P450scc (CYP11A1) initiates steroidogenesis by converting cholesterol to pregnenolone at the mitochondrial inner membrane, and the enzyme 3β-HSD binds with P450scc to form a complex inserted into the mitochondrial inner membrane of the mitochondria to synthesize progesterone. The mitochondrial intermembrane proton gradient is essential for the 3β-HSD activity. Mitochondrial dysfunction of the human granulosa cells may contribute to the decline of steroidogenesis.

Article Snippet: Granulosa cells were treated with a rabbit anti-FSHR primary antibody (cat# 3929, Sigma-Aldrich, Saint Louis, MO, USA) and then a goat anti-rabbit immunoglobulin G (IgG) secondary antibody (cat# ab6717, Abcam, Cambridge, UK).

Techniques: Binding Assay, Activity Assay